Gene Cloning

[Gene Cloning]

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....Call..  gene cloning
a new bio-technology company devoted to research. The goal is to provide 
scientific community with a wide variety of cell and molecular biology 
products that could enhance and accelerate research in cell & molecular 
genetics.

GENE CLONING OFFERS:
CONSTRUCT FRACTIONATED cDNA LIBRARY.
SCREENING GENE LIBRARY.
SUBTRACT LIBRARY.
SUB-CLONING.
EXPRESSION GENE IN VITRO.
SOUTHERN, NORTHERN, WESTERN BLOT.
DNA TESTING.
IN SITU HYBRIDIZATION.
IMMUNOCHEMITOHISTORY   &
PREPARING SOLUTIONS.(call today, deliver to lab. tomorrow)

GENE CLONING CONSULTED BY:
MOLECULAR BIOLOGISTS: Dr. L. Gan,  Dr. L. Li,  & Dr. Z. Wang.

2990 Richmond, Suite 330
Houston, TX 77098
Tel: (713) 522-5550
Fax: (713) 522-7447
Email: clonclon at hotmail.com


GENE CLONING OFFERS:

CUSTOM LIBRARY CONSTRUCTION
* ZAP expression & ZAP two Hybrids System vector 
   available, which contains prokaryotic & eukaryotic 
   promoters,unidirectional,specifically, the full length 
   cDNA can be cut out with restriction enzyme.
* Any selection of vectors.
* Any choice of mRNA priming method for cDNA 
   libraries.
* Quality control data provided with each library.
* Complete protocols and appropriate host strain(s) 
   sent with all custom libraries.


cDNA Library Vector	Cat #	Price $
Zap expression	     GC2001	4800
Zap II	             GC2002	4800
Lambda gt10	     GC2003	4000
Lambda gt11          GC2004	4000
Your choice	     GC200X	Negotiable

Genomic Library		
EMBL 3	             GC2011	4600
EMBL 4               GC2012	4600
EMBL3Sp 6/T7	     GC2013	4600
Cosmid pw 15	     GC2014	5100
Your choice	     GC201Y	Negotiable
PolyA RNA prep.	     GC2031	1500
Genomic DNA prep.    GC2032	1000

SUB-CLONING
* Any selection of vectors.
* Insertion region will be checked by sequencing.
* Insert size between 250-1500 BP are available.
   Insert size <250 or >1500 BP, the price are 
   negotiable.

Transcription Vector
	                End     Cat #	Price$
Bluescript	        sticky	GC1013	2000
Bluescript	        blunt 	GC1014	3000
pGEM- 3,5,7,9,11,13	sticky	GC1017	2000
pGEM- 3,5,7,9,11,13	blunt	GC1018	3000
pSp- 64,65,64poly(A)	sticky	GC1021	2000
pSp- 64,65,64poly(A)	blunt	GC1022	3000

Expression vector			
pGEMEX-1,2	  sticky	GC1025	2300
pGEMEX-1,2	   blunt	GC1026	3300
pSG5	          sticky	GC1027	2300
pSG5	           blunt	GC1028	3600
pSV-beta-Galactosidasesticky	GC1029	2300
pSV-beta-Galactosidase blunt	GC1030	3600
			
Specialized Vector  End	       Cat #	Price$
pALTER-1          sticky      GC1031	2300
pALTER-1	  blunt	      GC1032	3600
pGEM-Luc	  sticky      GC1033	2300
pGEM-Luc	  blunt	      GC1034	3600
pGEM-T	          sticky      GC1035	2300
pGEM-T	          blunt	      GC1036	3600

Reporter Vector			
pGLE2-system	sticky	      GC1037	2300
pGLE2-system	blunt	      GC1038	3600
 

OTHER SERVICES
 
	                   CAT #	PRICE$
DNA EXTRACTION
FROM SLIDE(QUALITY 
FOR PCR)	          GC1041	500
DNA TESTING		
 DOT BLOT(5-6 PROBES)     GC1045	500
 
RFLP   ( 3 PROBES )	
                          GC1046	600
    		
		
IN SITU HYBRIDIZATION
(FOR 20 SILDES.)	
                          GC1043	
                                        2000
GENE EXPRESSION
IN VITRO	          GC1040	NEGOTIABLE
SCREENING GENE
LIBRARY	                  GC1044	NEGOTIABLE

DNA SEQUENCING	          GC1040	NEGOTIABLE
GENE SPECIAL:
FRACTIONATED(10 frctions)    
cDNA library              GC1050      $ 20,000

REAGENT          PREPERATION
(* Autoclaved solution guaranted for one month)
Acrylamide gel sol. 500ml              Cat#        Price$
 for DNA sequence 5%-8%              GC3001        $20.00
 for gel shift                       GC3002        $20.00
 for protein analyses                GC3003        $20.00
(staking & seperating gel)
Cell transfection sol.               GC4001      $ 500.00
(free testing)
DNA loading buffer                   GC5001      $   5.00
EDTA 0.5M                   500ml    GC6001      $  35.00
Ethedium bromide 1%           1ml    GC5002      $   5.00
Hybridization Sol.        *  50ml    GC7001      $  30.00      
LB Broth                500m   *     GC8001      $  10.00  
LB Plate                100mm  *     GC8051      $   1.00
                        150mm  *     GC8052      $   3.00
Amp. Plate 100mm               *     GC8061      $   1.30
Amp. Plate  150mm              *     GC8062      $   3.00
Kan. Plate   100mm             *     GC8071      $   1.3
Kan. Plate    150mm            *     GC8072      $    3.0
X-gal Plate    100mm           *     GC8091      $    5.0
NaAc  3M              500ml    *     GC6002      $  30.00
NaCl  5M              500ml    *     GC6003      $  30.00
NZY Broth             500ml    *     GC8002      $  12.00
NZY Plate             100mm    *     GC8011      $   1.20
                      150mm    *     GC8012      $   3.60
PBS 1X                500ml    *     GC6004      $  10.00
PBS 10X               500ml          GC6005      $  10.00
Protein loading buffer  1ml          GC5003      $   5.00    
SDS 10%               500ml          GC6050      $  26.00
Southern Blot kit                    GC7050      $  30.00
 
50x TAE           500ml              GC6017      $   50.0
10x TBE           500ml              GC6008      $   45.0
20xSSC     500ml                     GC6006      $   25.0
20xSSC     500ml                     GC6007      $   25.0   
Screen library kit                   GC7070      $ 200.00
Transfer sol. for western  500ml     GC7060      $  20.00
Tris-HCl  (pH 7.0-8.0)  1M  500ml    GC6016      $  40.00

Additional special:
Special preperation                  GC9999           negotiable
Coming to lab. service               GC0922           negotiable
Trouble shooting                     GC0911           negotiable        

Please call (713) 522-5550
       Fax  (713) 522-7447
       Email clonclon at hotmail.com
For more information