In many experiments with DSP for protein cross-linking in Hepes buffer (20 mM, pH 7.9), DSP was quenched by 0.1M ethanolamine (1/10 ethanolamine 1M). But the pH of the assay becomes 11. So how that does not interfere with the following experiments (i.e. immunoprecipitation). Does anybody has the answer?. Thank you