quenching DSP

Joel Baguet Joel.Baguet at ens-lyon.fr
Mon Oct 20 12:50:48 EST 1997

In many experiments with DSP for protein cross-linking in Hepes buffer 
(20 mM, pH 7.9), DSP was quenched by 0.1M ethanolamine (1/10 
ethanolamine 1M). But the pH of the assay becomes 11. So how that does 
not interfere with the following experiments (i.e. 
immunoprecipitation). Does anybody has the answer?.
Thank you

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