quenching DSP

Joel Baguet Joel.Baguet at ens-lyon.fr
Mon Oct 20 12:50:48 EST 1997


In many experiments with DSP for protein cross-linking in Hepes buffer 
(20 mM, pH 7.9), DSP was quenched by 0.1M ethanolamine (1/10 
ethanolamine 1M). But the pH of the assay becomes 11. So how that does 
not interfere with the following experiments (i.e. 
immunoprecipitation). Does anybody has the answer?.
Thank you



More information about the Methods mailing list