genomic sequencing

Alex Dobrovic adobrovic at medicine.adelaide.edu.au
Tue Oct 21 02:57:13 EST 1997


In all the dicussion of genomic sequencing, it seems to have been
overlooked that this method can determine whether the cytosines are
methylated. The method that is now generally used utilizes
bisulfite-induced modification of genomic DNA and strand specific PCR. It
was developed by Marianne Frommer and more recently improved by Sue Clarke.

See

Frommer M, McDonald LE, Millar DS, Collis CM, Watt F, Grigg GW, Molloy PL,
Paul CL.A genomic sequencing protocol that yields a positive display of
5-methylcytosine residues in individual DNA strands.Proc Natl Acad Sci U S
A 1992 Mar 1;89(5):1827-1831

Clark SJ, Harrison J, Paul CL, Frommer M High sensitivity mapping of
methylated cytosines.Nucleic Acids Res 1994 Aug 11;22(15):2990-2997







Alexander Dobrovic, Ph.D.
Chief Scientist
Department of Haematology-Oncology
The Queen Elizabeth Hospital
Woodville, SA 5011, Australia

Affiliate Senior Lecturer
Department of Medicine
University of Adelaide

Tel 61-8-8222 6884
Fax 61-8-8222 6046





More information about the Methods mailing list