??Maleic acid buffers?????

Geoffrey Kidd GKidd at Aptagen.com
Tue Oct 21 14:43:53 EST 1997


Peter Schuchert wrote:
> 
> Martin Offterdinger wrote:
> >
> > Hi everyone!
> > Does anyone know what is the purpose of using maleic acid buffers for
> > RNA detection (esp. non-isotopic)? Does it in any way protect the RNA
> > from degradation?
> > MArtin
> 
> I think Maleic acid has been introduced by Boehriner as
> it dissolves their blocking reagent, which is
> otherwise quite recalcitrant.
> 
> cheers,
> Peter
> 
> -------------------------------------------------------
> PD Dr. P. Schuchert
> University Hospital INSEL
> Gastroenterology Unit  MEM-B814
> CH-3010 Bern Switzerland
> Tel 0041 31 632 84 92    Fax 0041 31 632 96 65
> -------------------------------------------------------


I believe Peter Schuchert is correct.  Boehringer's blocking reagent is 
the first (and so far only) place I had to use this buffer.

Geoffrey Kidd



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