??Maleic acid buffers?????
Geoffrey Kidd
GKidd at Aptagen.com
Tue Oct 21 14:43:53 EST 1997
Peter Schuchert wrote:
>
> Martin Offterdinger wrote:
> >
> > Hi everyone!
> > Does anyone know what is the purpose of using maleic acid buffers for
> > RNA detection (esp. non-isotopic)? Does it in any way protect the RNA
> > from degradation?
> > MArtin
>
> I think Maleic acid has been introduced by Boehriner as
> it dissolves their blocking reagent, which is
> otherwise quite recalcitrant.
>
> cheers,
> Peter
>
> -------------------------------------------------------
> PD Dr. P. Schuchert
> University Hospital INSEL
> Gastroenterology Unit MEM-B814
> CH-3010 Bern Switzerland
> Tel 0041 31 632 84 92 Fax 0041 31 632 96 65
> -------------------------------------------------------
I believe Peter Schuchert is correct. Boehringer's blocking reagent is
the first (and so far only) place I had to use this buffer.
Geoffrey Kidd
More information about the Methods
mailing list