Funny bands

[Geoffrey Kidd]

Prolonged alkaline lysis can denature plasmids somehow, according to my 
Qiagen Mini-Prep Kit's manual (no affiliation).  They show a gel with a 
faster band just as you describe, that is restriction-resistant.

Geoffrey Kidd

Ed Schwartz wrote:
> 
> Hi,
> Ive been eluting DNA fragments from agarose gels using
> NaI and silica-based spin columns (GibcoBRLs GlassMAX).
> Does anyone know where the additional, faster-migrating bands
> come from that are sometimes present after elution?
> 
> Ed Schwartz
> edward=schwartz at rz.hu-berlin.de
> Institut fuer Biologie/Mikrobiologie
> Humboldt-Univeritaet Berlin