GKidd at Aptagen.com
Tue Oct 21 14:34:18 EST 1997
Prolonged alkaline lysis can denature plasmids somehow, according to my
Qiagen Mini-Prep Kit's manual (no affiliation). They show a gel with a
faster band just as you describe, that is restriction-resistant.
Ed Schwartz wrote:
> Ive been eluting DNA fragments from agarose gels using
> NaI and silica-based spin columns (GibcoBRLs GlassMAX).
> Does anyone know where the additional, faster-migrating bands
> come from that are sometimes present after elution?
> Ed Schwartz
> edward=schwartz at rz.hu-berlin.de
> Institut fuer Biologie/Mikrobiologie
> Humboldt-Univeritaet Berlin
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