Help - PCR

Geoffrey Kidd GKidd at Aptagen.com
Tue Oct 21 14:10:37 EST 1997


Well-to-well temperature variations come to mind.  Is it reproducible in 
those wells?

G. Kidd

Doug Jones wrote:
> 
> I have been having some unusual results from running an RT-PCR for
> murine IL-6.  After (supposed) optimization, I ran 3 tubes using the
> control cDNA, using the same mix.  In one tube, I had a good band, the
> other two gave no results.  Anyone have any suggestions?
> 
> Douglas Jones
> jonesd at telenet.net



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