DIG Northern blot

Susanna Malmström pbio-sma at MERKURIUS.LU.SE
Tue Oct 21 09:58:23 EST 1997


Hi,

I want to do non-radioactive Northerns using the DIG-system. With
DNA-probes, like I have, Boehringer recommends a "high SDS buffer" with 7%
SDS, 50% formamide, 5xSSC and some other stuff. For preparation they say to
do a 30xSSC stock solution, otherwise the volume is too big. It's just that
30xSSC is very difficult to get into solution! Only after warming to almost
boiling, but it falls out again when it's cooling down.
WHAT TO DO? Has anyone had the same experience?

Susanna





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