vacuum infilatration of plant tissue

[Geoffrey Kidd]

Mike Allen wrote:
> 
> Hello:
> 
> I am trying to fix plant buds by chemical fixation. I am having troubles
> because the buds float in the fixative. I have tried to keep them in the
> solution using a piece of mesh but am concerned that it takes a long
> time to get the tissue into the fixative. Does anyone have a homemade
> solution or a suggestion as to how solve this?
> thanks for your help. Please reply to the email address below. I will
> repost answers.
> Chenhong Zhang
> c4zhang at scimail.uwaterloo.ca


Mesh sounds like a good idea to me - I'm not clear on your concern with 
that method.  Are you worried that the fixative is taking too long to 
penetrate?

G. Kidd