gene expression

Xiao-hua Xue xxue at
Wed Oct 22 16:11:32 EST 1997


  I have been trying to express a gene from cDNA library using xenopus
oocytes and it hasn't got any expression yet after I tried twice. There is
about 200 nucleotides at 5'-terminal before the start codon. Could this
(long non-coding sequence) be one of the reasons for no expression? 

  Any help will be really appreciated.


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