timt at itsa.ucsf.edu
Thu Oct 23 02:38:23 EST 1997
Top in my head I can not think of particular refs. I am doing a similar
kind of exp. Two potential problems: one is the ultra-hi expressors
(actin etc) will shadow/mask the signals you are looking for, the other
being that expression of the mRNAs of your interest needs to be
sufficiently hi to give a detectable signal.
In article <Pine.A22.214.171.1241022113120.64552B-100000 at lucia.u.arizona.edu>,
Harold E Smith <hes at U.Arizona.EDU> wrote:
> I would like to probe a blot of cDNA clones with mRNA isolated from two
> different tissues to identify clones that are differentially expressed.
> I'm fairly certain it's been done before, but I can't find a reference.
> Would someone be so kind as to point me in the right direction?
> Thanks in advance,
> Harold Smith
More information about the Methods