looking for F'(lacZdeltaM15) without lacI

Pascal Mertens Pascal.Mertens at fundp.ac.be
Thu Oct 23 09:51:55 EST 1997


In article (Dans l'article) <344edae7.76495314 at news.uvic.ca>,
gstuart at uvic.ca wrote (écrivait) :

> Does anyone have a strain harboring an F' plasmid with the
> lacZdeltaM15 gene but WITHOUT the lacI gene? A plasmid that totally
> lacks the lacI gene sequence  would be the most useful to me; also, if
> the plasmid expressed tetracycline or ampicillin resistance, that
> would be ideal!  (I can't use proAB to maintain the plasmid.)

Look at the DH5alphaF' strains (GibcoBRL)
there is one with lacIq and one without

Pascal

-- 
Pascal Mertens
Laboratoire d'Immunologie et Microbiologie
URBM-FUNDP
61 Rue de Bruxelles
5000 Namur, BELGIUM
Phone: 32 81 724438
Fax: 32 81 724420



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