looking for F'(lacZdeltaM15) without lacI

Pascal Mertens Pascal.Mertens at fundp.ac.be
Thu Oct 23 09:51:55 EST 1997

In article (Dans l'article) <344edae7.76495314 at news.uvic.ca>,
gstuart at uvic.ca wrote (écrivait) :

> Does anyone have a strain harboring an F' plasmid with the
> lacZdeltaM15 gene but WITHOUT the lacI gene? A plasmid that totally
> lacks the lacI gene sequence  would be the most useful to me; also, if
> the plasmid expressed tetracycline or ampicillin resistance, that
> would be ideal!  (I can't use proAB to maintain the plasmid.)

Look at the DH5alphaF' strains (GibcoBRL)
there is one with lacIq and one without


Pascal Mertens
Laboratoire d'Immunologie et Microbiologie
61 Rue de Bruxelles
5000 Namur, BELGIUM
Phone: 32 81 724438
Fax: 32 81 724420

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