Elimination of M13?

Troels Wind wind at biobase.dk
Thu Oct 23 06:13:59 EST 1997


Paul R. Rohde (P.Rohde at mailbox.uq.edu.au) wrote:
: hessling at toxi.uni-wuerzburg.de (=?ISO-8859-1?Q?Jutta_He=DFling?=) writes: > Hi,
: > I am working with M13 phage and wonder how I could
: > decontaminate used plasticware. Is 70% ethanol
: > sufficient?
: > 
: > Thanks,
: > Jutta.

: Ambion's RNaseZap cleans plasticware (and pippettes) pretty well.
: Rinse twice with water after.

We do the following to kill M13:

Soak 10' in 1%SDS, 0.2M NaOH
Rinse 3x with water
Rinse 1x in EtOH
Rinse 3x with water.

/Troels



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