Saturated O2 concentration in Tris buffer?
Jorge L. Galazzo
jlg at alumni.caltech.edu
Thu Oct 23 16:54:37 EST 1997
In article <62nuq5$6v3$1 at lyra.csx.cam.ac.uk>,
Justin Powell <jacp1 at mole.bio.cam.ac.uk> wrote:
>I need to know the concentration of oxygen in air saturated 50mM Tris-HCl
>pH7.6 buffer at 25C in order to interpret some oxygen electrode data I
>have. Does anyone have a value (preferably with reference) for this? I
>think the effect of salts on solubilities at low concentrations may be
>additive so I guess values for different concentrations could be scaled
>to 50mM?
>
>Justin Powell
>Dept. Genetics
>Cambridge
>UK
>
Justin,
Data taken from "Biochemical Engineering Fundamentals" 2nd ed., Bailey and
Ollis. p463 (1986)
NaCl (mM) 0 500 1000 2000
O2 (mmol/L) 1.26 1.07 0.89 0.71
This is not Tris, but hopefully will help.
Jorge
jlg at alumni.caltech.edu
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