Size fractionating before making cDNA library
tyarovin at blue.weeg.uiowa.edu
Fri Oct 24 16:29:31 EST 1997
This is a novice's quiestion.
I am trying to make a cDNA library with Lambda ZAP II vector. I need to
size fractionate cDNA, which was ligated to adaptors, before ligating to
vector arms. I did not label the cDNA and have less than 300 ng of cDNA,
that's why I cannot monitor fractions collected from Sepharose column
neither with counter nor on a gel. I have a stupid idea to add some
markers (Lambda or Phix 174 digests) to my sample to monitor the fractions
on agarose and/or polyacrylamide gel.
So, your critique is wellcome.
Timur Yarovinsky, Ph.D., M.D.
Oakdale Research Park, B141 MTF
2501 Crosspark Road
Coralville IA 52241-8802
tel. (319) 335-4608
fax (319) 335-4347
e-mail: timur-yarovinsky at uiowa.edu
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