cDNA synthesis to study mRNA

Pamela Lagali plagali at gpu.srv.ualberta.ca
Fri Oct 24 16:27:53 EST 1997


Hello everyone,

I am interested in studying the mRNA for a particular protein by
sythesizing the corresponding cDNA and analyzing that by Southern blotting
and sequencing.  Has anyone else out there attempted this successfully? 
For various reasons, RT-PCR and cDNA library construction (i.e. common
methods for mRNA analysis involving cDNA synthesis)  are procedures I do
not want to undertake at this time. 

Any input would be much appreciated.

Thanks,

Pam



Pamela Lagali
Dept. of Cell Biology and Anatomy
University of Alberta
Edmonton, Alberta, Canada
T6G 2H7



More information about the Methods mailing list