cDNA synthesis to study mRNA

Pamela Lagali plagali at
Fri Oct 24 16:27:53 EST 1997

Hello everyone,

I am interested in studying the mRNA for a particular protein by
sythesizing the corresponding cDNA and analyzing that by Southern blotting
and sequencing.  Has anyone else out there attempted this successfully? 
For various reasons, RT-PCR and cDNA library construction (i.e. common
methods for mRNA analysis involving cDNA synthesis)  are procedures I do
not want to undertake at this time. 

Any input would be much appreciated.



Pamela Lagali
Dept. of Cell Biology and Anatomy
University of Alberta
Edmonton, Alberta, Canada
T6G 2H7

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