cDNA synthesis to study mRNA
plagali at gpu.srv.ualberta.ca
Fri Oct 24 16:27:53 EST 1997
I am interested in studying the mRNA for a particular protein by
sythesizing the corresponding cDNA and analyzing that by Southern blotting
and sequencing. Has anyone else out there attempted this successfully?
For various reasons, RT-PCR and cDNA library construction (i.e. common
methods for mRNA analysis involving cDNA synthesis) are procedures I do
not want to undertake at this time.
Any input would be much appreciated.
Dept. of Cell Biology and Anatomy
University of Alberta
Edmonton, Alberta, Canada
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