his tags

Vickers Burdett burdett at ABACUS.MC.DUKE.EDU
Fri Oct 24 15:14:21 EST 1997

I have been having a lot of trouble getting my his-tagged protein to
bind well to any metal affinity column and see about 70% of the material
in the 'pass-thru' fraction.  My protein is poorly made under any
condition and there is not a lot of material in inclusion bodies for
example.  Are there any suggestions out on the net?

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