Calcium and GTP

Philip Aisen aisen at bloom.aecom.yu.edu
Fri Oct 24 14:09:37 EST 1997


When we add 30mM calcium chloride to 5mM GTP in Mg/EGTA/sucrose/PIPES
buffer, pH 7.4, a heavy white ppt. develops.  No precipitate develops
with ATP under similar circumstances.  No free phosphate is evident by
NMR spectroscopy.  

Does anyone have an explanation?

Thanks.

Philip Aisen



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