A short linker

Yoram Gerchman yoramg2 at LEONARDO.LS.HUJI.AC.IL
Sun Oct 26 04:10:32 EST 1997


Greetings. I'm tryng to insert a short linker (about 45 nt) into a
restriction site in the middle of a gene. Now, my idea was to creat the
linker in such a way that it will leave cohesive ends that fit the RE.

somthing like

	AAACCCCCCCCCCCC	
	   ||||||||||||
	   GGGGGGGGGGGGTTT

Anyone tried this?
.........................................................................
Yoram Gerchman
Division of Microbial & Molecular Ecology
The Institute of Life Sciences
Hebrew University of Jerusalem
Givat Ram, Jerusalem 91904, Israel
Tel: 972-2-6585175




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