NASBA

rabc010 at S1.CXWMS.AC.UK rabc010 at S1.CXWMS.AC.UK
Sun Oct 26 13:29:32 EST 1997


WHAT IS NASBA (Nucleic acid sequence-based amplification): also 
              known as self-sustained sequence replication (3SR).
The (NASBA or 3SR) is an isothermal method of nucleic acid
amplification and an alternative to PCR. A target nucleic acid sequence
can be amplified exponentially in vitro using two enzymes: reverse
transcriptase (RT) and a DNA-dependent RNA polymerase (RNAP).

References:

1. Compton J, 1991. Nucleic acid sequence-based amplification. 
   Nature 350(6313), 91-92. 
2. Malek L, Sooknanan R, Compton J, 1994. Nucleic acid 
   sequence-based   amplification (NASBA). 
   Methods Mol Biol;28:253-260. 
3. van Gemen B, v d Wiel P, van Beuningen R, Sillekens P, Jurriaans S,
   Dries C, Schoones R, Kievits T(1995).
   The one-tube quantitative HIV-1 RNA NASBA:precision, accuracy, 
   and  application.
   PCR Methods Appl;4(4):S177-S184.
4. Romano JW, Williams KG, Shurtliff RN, Ginocchio C, Kaplan M (1997).
   NASBA technology: isothermal RNA amplification in qualitative 
   and quantitative diagnostics. Immunol Invest;26(1-2):15-28.



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