Why has glucose to be autoclaved seperately?

Hiranya Roychowdhury hroychow at NMSU.EDU
Mon Oct 27 10:41:22 EST 1997


At 03:20 PM 10/27/97 -0800, Frederik Boernke wrote:
>Hi!
>
>In any protocol where sterile glucose is needed,  yeast media for 
>instance, it tells me to autoclave the glucose seperately and to add
>it afterwards. What happens to it when I add it before?
>
>Cheers
>Ricky
>

Sugars char (caramelize) very readily in the presence of salts. However,
glucose is normally "cold-sterilized".




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