Why has glucose to be autoclaved seperately?
hroychow at NMSU.EDU
Mon Oct 27 10:41:22 EST 1997
At 03:20 PM 10/27/97 -0800, Frederik Boernke wrote:
>In any protocol where sterile glucose is needed, yeast media for
>instance, it tells me to autoclave the glucose seperately and to add
>it afterwards. What happens to it when I add it before?
Sugars char (caramelize) very readily in the presence of salts. However,
glucose is normally "cold-sterilized".
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