Activity restored by mixing recombinant domains?
lloyd.graham at molsci.csiro.au
Mon Oct 27 23:57:24 EST 1997
I'm looking for any precedents where the domains of a monofunctional
enzyme have been expressed separately as inactive proteins, but when mixed
together they associate to form a complex with significant enzymatic
The closest I have got to an example is mouse creatine kinase, where
independent expression of the two domains gives inclusion bodies but
co-expression yields active soluble enzyme [Gross et al., 1996: Prot. Sci.
If anybody can provide instances where purified inactive domains
complement in vitro, I would be very grateful.
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