his tags

[Alejandro Krimer]

Dear Burdett:
		What's about the conditions to bind your protein to
these resins? Do you use Urea or same chaotropic agent?
And finally, I have used Ni-NTA resin from Qiagen, It works fine

				Alejandro Krimer 

> ----------
> From:
> burdett at ABACUS.MC.DUKE.EDU[SMTP:burdett at ABACUS.MC.DUKE.EDU]
> Sent: 	Friday 24 de October de 1997 17:14
> To: 	methods at net.bio.net
> Subject: 	his tags
> 
> I have been having a lot of trouble getting my his-tagged protein to
> bind well to any metal affinity column and see about 70% of the
> material
> in the 'pass-thru' fraction.  My protein is poorly made under any
> condition and there is not a lot of material in inclusion bodies for
> example.  Are there any suggestions out on the net?
>