large DNA plasmid prep

[gj at nospampobox4.stanford.edu]

Lakshman Ramamurthy PhD wrote:
> 
> Netters:
> 
> I have tried unsuccessfully to make a Qiagen large prep of a 23 kb
> plasmid DNA.. Do you have any suggestions to get around this problem. I
> need about 100 micrograms of DNA
> Thanks
> LR

Hi When upping te=he volume of the bacterial culuture you need to lyse
the cells alittle more rigorously. Also large plasmids are notoriously
hard to grow. You might concider some of the new strains of bacteria
that are specifically designed to increase the yield of large plsmids