Looking for a pBluescript with KAN selection or...

Frederik Boernke boernke at ipk-gatersleben.de
Mon Oct 27 18:17:36 EST 1997

S. Findley wrote:
> Has anyone out there constructed a pBluescript with Kanamycin resistance
> instead of Ampicillin?
> There is a pesky Eam1105I/AhdI restriction site at position ~2046 in the
> Amp resistance gene in pBS that I either need to mutate in the Amp or
> replace the cassette with th Kanamycin resistance gene.
> Any tips appreciated.
> (Does anyone out there know how companies selling T-vectors are making the
> 3' T-overhangs efficiently? I was planning on making the T-vector with 2
> AhdI/Eam1105I resitriction sites in the polylinker til I noticed the site
> in the Amp resistance gene. Perhaps a company has already mutated that
> site?)
> -Thanks

> Seth D. Findley               polliwog at u.washington.edu
> Department of Biochemistry    Phone: 206-543-1710
> Health Sciences Building J579
> University of Washington, Box 357350, Seattle, WA 98195


Stratagene has this pBK-CMV which actually is a modified pBluescript
with a CMV promotor, besides the plac, and a kan resistence.



Frederik Boernke
Research Group of  Molecular Plant Physiologie
Institute for Plant Genetics and Crop Plant Research (IPK)
Corrensstr. 3
06466 Gatersleben
Tel.  039482 -5 321
Fax. 039482 -5 515
e-mail: boernke at ipk-gatersleben.de

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