A short linker

Marie-Claude Serre serre at lebs.cnrs-gif.fr
Mon Oct 27 10:58:18 EST 1997


yes, I did it a few years ago, with a blunt on one side and a cohesive
end on the other. Briefly, the following procedure was used:

- cut the target plasmid with the relevant enzymes
- purify the "big" fragment
- hybridize the oligos
- ligate the ds oligo (about 50 pmoles) with the plasmid fragment
- purify the ligation product, phosphorylate and religate.
- transformation



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