tyrosine kinase assay

Rizwan Haq haqr at oci.utoronto.ca
Mon Oct 27 18:36:19 EST 1997


There was an ELISA-based method discussed in a recent issue of Biotechniques
that was used for this purpose. It involves using phospho-specific
antibodies. If you can't find it, I'll try to see if I could dig it out of
my stack of papers ;)

Rizwan


Grace Nakayama wrote in message
<01bcdf2a$f4188780$8d01a8c0 at osmium.irori.com>...
>I would like to learn about methods for measuring autophosphorylation of
>various receptor tyrosine kinases. I am especially interested in methods
>which would be suitable for drug screening, but all responses are welcome.
>I am already familiar with protocols which measure phosphate incorporation
>into substrates such as poly[glu,tyr].
>
>Thank you very much
>
>-Grace
>
>----------------------------------------------------
>Grace Nakayama, Ph.D.
>IRORI
>11025 N. Torrey Pines Road
>La Jolla, CA 92037
>1-619-546-1300 tel
>1-619-546-3083 fax
>nakayama at irori.com
>





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