Internal Standard for Northern

Robert W. St. G. Fisher, IV fisher at
Mon Oct 27 17:00:28 EST 1997

The main drawback that comes to mind is how damn _strong_ the signal
is.  The signal from the 18S will drown out everything else.  However,
if you are stripping and then reprobing a blot, you can do all the
probing for weak stuff first, and then do your 18S hybridization.

Of course, the flip side of this is that you can label up some 18S
probe, then split it between several blots....

Robert Fisher
fisher at

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