Cloning Vector effect ?
GKidd at Aptagen.com
Mon Oct 27 13:36:10 EST 1997
Malini Madiraju wrote:
> Hi Netters,
> I have a unique problem. I cloned a 5 kb piece of DNA in pUC18 and the
> same one in pZErO vector. I get deletions in the insert cloned in pZErO
> but not in the one cloned in pUC18. I would prefer to use pZErO and not
> get any deletions. Did anyone else observe this or have a clue to what
> might be happening ? I appreciate any comments/suggestions.
Is this repeatable?
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