large DNA plasmid prep

plxsrt at plxsrt at
Mon Oct 27 14:58:54 EST 1997

Lakshman Ramamurthy PhD <lr50916 at> wrote:


>I have tried unsuccessfully to make a Qiagen large prep of a 23 kb
>plasmid DNA.. Do you have any suggestions to get around this problem. I
>need about 100 micrograms of DNA

The bit in the manual about using larger volumes of culture seems to
be critical for large plasmids/cosmids.  I'd check how well your
plasmid grows using a crude prep before using the Qiegen.  If it
doesn't grow well then bump up the culture volume.

Simon T

More information about the Methods mailing list