A short linker

plxsrt at pln1.nott.ac.uk plxsrt at pln1.nott.ac.uk
Mon Oct 27 14:48:56 EST 1997


yoramg2 at LEONARDO.LS.HUJI.AC.IL (Yoram Gerchman) wrote:
<cut>
I'd go for the "unphosphorylated" adaptor/linker and "phosphorylated"
vector.  

You can easily screen for oligo insertion if make the oligo so that
when it ligates into the restriction site it doesn't reform the
restriction sites at the junctions.  This way you can screen for oligo
insertion by looking for those clones that have lost the insertion
restriction site.   (I'd then confirm by sequencing).


Good luck


Simon T





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