cDNA synthesis to study mRNA

[Geoffrey Kidd]

Pamela Lagali wrote:
> 
> Hello everyone,
> 
> I am interested in studying the mRNA for a particular protein by
> sythesizing the corresponding cDNA and analyzing that by Southern blotting
> and sequencing.  Has anyone else out there attempted this successfully?
> For various reasons, RT-PCR and cDNA library construction (i.e. common
> methods for mRNA analysis involving cDNA synthesis)  are procedures I do
> not want to undertake at this time.
> 
> Any input would be much appreciated.
> 
> Thanks,
> 
> Pam
> 
> Pamela Lagali
> Dept. of Cell Biology and Anatomy
> University of Alberta
> Edmonton, Alberta, Canada
> T6G 2H7


One method is actually a service my company offers, so I can't make the 
usual "No affiliation" disclaimer.  We construct genes from scratch.  
This allows various modifications to be built into the construct, such 
as codons optimized for a particular host, the addition/subtraction of 
regulatory regions, etc.  If you'd like further info, I'll have some 
sent to you, or you can visit our web site at www.aptagen.com.

Geoffrey Kidd, Ph.D.
Technical Services Manager
Aptagen, Inc.