Why has glucose to be autoclaved seperately?

Christoph Winter chw at sirius.mgen.uni-heidelberg.de
Tue Oct 28 15:08:30 EST 1997


Hi!

>In any protocol where sterile glucose is needed (...) it tells me to autoclave the glucose seperately ...

At higher temperatures some of the glucose is reacting with
aminofunctions (proteins, peptides etc.) in a socalled Maillard reaction.
The products of this reaction are not uniform and usually from darker
color - your medium gets a dark brown touch like caramel. I Hope this
helped.


Regards


Christoph



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