In vivo biotinylation

D. KIM dkim at NMSU.Edu
Tue Oct 28 18:29:19 EST 1997


Hello:

I have read a bit about in vivo biotinylation of proteins by fusing the
desired gene onto a fragment of the biotin carboxy-carrier protein gene.
The resulting fusion protein is biotinylated in vivo, and is suitable for
labeling with avidin-conjugates or purification in avidin solid matrices.

The gene fragment seems to be rather large (about 85 aa), and I was
wondering if one could simply tag the protein with some kind of minimal 
biotinylation site?  Has anyone done this?

Daniel Kim
dkim at nmsu.edu



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