fractionation of large complexes

Michael Ladomery mladomery at hgu.mrc.ac.uk
Wed Oct 29 17:56:38 EST 1997


hello folks,
my protein of interest is present in large nuclear complexes, in fact
probably spliceosomes and related structures. What sort of fractionation
materials and techniques can I try, to take a nuclear extract and
fractionate? I have tried with success, Nycodens runs, and Glycerol and
Sucrose, but these are not good for >200S complexes. Are there special
resins, for example... any help much appreciated

mladomery at hgu.mrc.ac.uk




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