Transfection of smooth muscle cells
klenchin at facstaff.REMOVE_TO_REPLY.wisc.edu
Wed Oct 29 17:02:21 EST 1997
In article <34570505.AD38EB69 at nott.ac.uk>, Simon.Dawson at nott.ac.uk wrote:
: Has anybody out there had need to perform transfections on
: primary smooth
:muscle cell cultures? We are trying to do just that and so far have had little
: If you have any info. on the best transfection reagent to use and any
:important details for doing this successfully, I'd love to hear about it!
Really can't go wrong with electroporation. Even if there is some
"reagent" that'll give better efficiency (may or may not), chances are
you'll be done with electroporation before you have a chance to try half
of the lipids available on the market.
More information about the Methods