In article <3457A7F4.2A54 at ipk-gatersleben.de>, Frederik Boernke <boernke at ipk-gatersleben.de> wrote: > What does sorbitol in yeast SD medium ? Sorbitol acts as osmotic stabilizer and is added to the media/solutions, whenever the danger of lysis is eminent (such as downstream processing of spheroplasts). regards, V.