In vivo biotinylation

Dr. Duncan Clark duncan at
Thu Oct 30 04:20:55 EST 1997

In article <635sgf$kg at bubba.NMSU.Edu>, "D. KIM" <dkim at NMSU.Edu> writes
>The gene fragment seems to be rather large (about 85 aa), and I was
>wondering if one could simply tag the protein with some kind of minimal 
>biotinylation site?  Has anyone done this?

There are a number of these around. Try looking for a STREP tag plasmid
I think sold by Biometra and I think published by Skerra et al (totally
from memory so I may be wrong). Alternatively try a paper by Peter
Schatz of Affymax, maybe 4 years or so ago on consensus peptide
libraries specifying biotinylation. I don't think the STREP tag is
actually biotinylated in vivo but is a peptide that bind sto avidin and
strepatvidin with high enough affinity to be eluted later without harsh

The problem with being on the cutting edge is that you occasionally get 
sliced from time to time....

Duncan Clark
DNAmp Ltd.
TEl/FAX 01252376288

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