his-tagged protein purification from yeast

Vladimir Svetlov svetlov at oncology.wisc.edu
Thu Oct 30 16:13:55 EST 1997


I'd greatly appreciate a protocol (or a pointer to such protocol) for
purification of his-tagged proteins from yeast (S. cerevisiae) [both native
and denaturing conditions] using Ni-NTA columns (Qiagen). Comments and tips
derived from the personal experience will be appreciated even greater than
greatly.
Regards,
V.



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