subcloning of rearrangement-prone DNA
kjia at BLUE.WEEG.UIOWA.EDU
Thu Oct 30 13:27:27 EST 1997
I have had problems with subcloning one piece of mammalian DNA . With
DH5alpha cells, I got apparently rearrangement of that DNA when ligated
to pBluescript. Now I tried Sure cells bought from Stratagene but have
not got any colonies (almost no background blue colonies as well).
However, pUC18 control plasmid did give some colonies.
Any ideas what I can try next?
More information about the Methods