subcloning of rearrangement-prone DNA

Hongyan Liu kjia at BLUE.WEEG.UIOWA.EDU
Thu Oct 30 13:27:27 EST 1997


Everyone,

I have had problems with subcloning one piece of mammalian DNA . With
DH5alpha cells, I got apparently rearrangement of that DNA when ligated
to pBluescript. Now I tried Sure cells bought from Stratagene but have
not got any colonies (almost no background blue colonies as well).
However, pUC18 control plasmid did give some colonies.

Any ideas what I can try next?

Thanks.

HL



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