labeling probes

CHANG SHU WANG aae138 at
Thu Oct 30 11:51:21 EST 1997

I want to have a synthesis of radiolabed probes by random primer extension
for a double-stranded DNA insert (1,7 Kd) in my Northern work. I notice
some difference between the protocol of Current Protocol and Maniatis'
one. In Matiatis, 200 ng DNA with 75 ng random primers in 10 ul
reaction volume, while in Current Protocol, 30-100 ng DNA with 1000-5000
ng random primers in 25 final volume. 

Which one is better? Any suggestion will be appreciable.


Changshu Wang

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