?? PCR with biotinylated nucleotides

Dale Kunde Dale.Kunde at appbio.utas.edu.au
Thu Oct 30 23:29:00 EST 1997


I read in Methods in Molecular Biology Vol 15 PCR Protocols edited by
B.A. White about direct incorporation of digoxigenin labelled
nucleotides during PCR. Could anyone tell me if this technique is
possible for biotinylated nucleotides. If anyone has tried it, could you
let me know if it's possible before I spend time and $ playing around
with it.

Thanks in advance

Dale Kunde
School of Biomedical Science
University of Tasmania
Tasmania Australia
Dale.Kunde at utas.edu.au



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