RNA electrophoresis!!

[Shelley Cole]

At 06:46 PM 10/31/97 GMT, you wrote:
>Han <huangz at hotmail.com> wrote:
>> Dear netters,
>
>> I encountered a problem in RNA formaldehyde electrophoresis: I added EB 
>> in my formaldehyde agarose gel(final concentration 50ug/100ml) and run 
>> gel for 2 hours. Amazingly, when I visualize gel under UV lamp I found 
>> very high staining background which evenly distributed and composed 
>> almost 2/3 area of gel starting.......
>
>I put ethidium bromide in the loading buffer. Am I the only one?
>
>Trei
>
>
>

No.  I also put ethidium bromide in the loading buffer for my formaldehyde gels.

Shelley



Shelley Cole, Ph.D.
SW Foundation for Biomedical Research
San Antonio, TX
scole at darwin.sfbr.org
(210)645-3488