T vector vs. RE

[Promega]

At Promega, our testing indicates that the T- and A-overhangs are very
robust (being,
after all, covalent bonds). After week-long incubation at elevated
temperatures, cloning
efficiencies remain as high as our QC specifications. Same holds true for
multiple
freeze-thaw cycles on both vector and insert.
The "fragility" of T- and A-overhangs generally is seen when a user
inadvertently
introduces nucleases during routine handling.
Hope you find this helpful.

Yoram Gerchman <yoramg2 at LEONARDO.LS.HUJI.AC.IL> wrote in article
<Pine.SGI.3.94.971030135636.13387A-100000 at leonardo.ls.huji.ac.il>...
> Greeting Netnauts.
> I was wondering, why are the T's in the ends of T vector and the A in the
> ends of a PCR product so fragile while the cohesive ends of RE digestion
> are so stable?
> 
> .........................................................................
> Yoram Gerchman
> Division of Microbial & Molecular Ecology
> The Institute of Life Sciences
> Hebrew University of Jerusalem
> Givat Ram, Jerusalem 91904, Israel
> Tel: 972-2-6585175
> 
> 
>