Any idea why?

Geoffrey Kidd GKidd at
Fri Oct 31 11:21:01 EST 1997

Majde wrote:
> I doning in-situ hybridization in section detection MYC mRNA. I am
> geting signal in the cytoplasm as well as spot in the nucleus of some
> cells. Is any body know why I am getting signal in nucleus if we know
> that the mature mRNA in the cytoplasm. Beside that the probes I am
> used is olignucleotide and design specifically for mRNA only. Any help
> will be appreciated.
> Majde

How is it designed specifically for mRNA only?  Is there no chance of 
cross-hybridization to the nuclear form?

Geoffrey Kidd

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