Activity restored by mixing recombinant domains

[Aydemir Akin, DVM]

Regarding: colossus... (s535290 at aix1.uottawa.ca) wrote:

I think the following link might help.  It is about a Novagen product,
RNase-S which was separated in to two domains.  You clone a fragment into
their vector which contains S-Tag and express the protein (S-Tag) in the
presence of the other protein complementing the enzymatic activity.  You
can check the link.
http://www.novagen.com/1inno2.html

I don't have any associations with this company except I use their vectors.

-- 
Aydemir Akin, DVM
Animal Disease Diagnostic Lab
Purdue University
West Lafayette, IN 47907-1175

Phone: 765-494-1040
E-mail: akin at purdue.edu