transformation proplems with XL-1 Blue

agruendling at biochem.tamu.edu agruendling at biochem.tamu.edu
Mon Sep 1 18:09:23 EST 1997


A plasmid purified from XL-1Blue transforms other E.coli K12 strains
very poorly. If we transform the plasmid back into Xl-1 Blue, we get
normal transformation efficiency. So it seems that the modification
system of XL-1Blue does not work very well and so we can't get the
plasmid into a strain that is r+  m+.  Has anyone had problems
transforming other strains with plasmids purified from XL-1 Blue????? 





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