Transformation problems with XL-1 Blue

agruendling at agruendling at
Mon Sep 1 16:22:08 EST 1997

A plasmid purified from XL-1Blue transforms other E.coli K12 strains
very poorly. If we transform the plasmid back into Xl-1 Blue, we get
normal transformation efficiency. So it seems that the modification
system of XL-1Blue does not work very well and so we can't get the
plasmid into a strain that is r+  m+.  Has anyone had problems
transforming other strains with plasmids purified from XL-1 Blue????? 

More information about the Methods mailing list