Blotting of Coomassie stained gels possible?

Howard Petrie h-petrie at ski.mskcc.org
Tue Sep 2 11:35:30 EST 1997


("Andreas M. Stolpe") wrote:

> Just a short question: Is Western Blotting of Coomassie stained PAGE
> possible? Do you have some recipes or suggestions?

Theoretically, no.  FMC now sells a kit based on a fluorescent (Sybr) dye
that supposedly allows transfer and WB detection after staining.  Imaging
is UV based (standard 300nm box).  We haven't tried it, but FMC stuff is
usually pretty reliable.



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