Blotting of Coomassie stained gels possible?
h-petrie at ski.mskcc.org
Tue Sep 2 11:35:30 EST 1997
("Andreas M. Stolpe") wrote:
> Just a short question: Is Western Blotting of Coomassie stained PAGE
> possible? Do you have some recipes or suggestions?
Theoretically, no. FMC now sells a kit based on a fluorescent (Sybr) dye
that supposedly allows transfer and WB detection after staining. Imaging
is UV based (standard 300nm box). We haven't tried it, but FMC stuff is
usually pretty reliable.
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