How to remove DNA from RNA for RT-PCR

Marcos Malumbres malumm01 at
Wed Sep 3 17:09:44 EST 1997

I've treated my RNA with RNase-free DNase I (following Sambrok et al.,
and Ausubel et al. procedures) at least 3 consecutive times and I still
get DNA-generated bands in my RT-PCR. Does anybody know a method to
absolutely remove DNA contamination from RNA preparations?


Marcos Malumbres

Dep. of Pathology, New York University Medical Center
550 First Ave. New York, NY10016
Tel. (212)2635342, Fax (212)2638211
email:malumm01 at


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