Intron Processing

Jill Kellogg jkellogg at epitope.com
Wed Sep 3 15:02:22 EST 1997


Dicot introns would be likely to work in other dicots.  A sweet potato intron 
(STLS1 intron 2) has worked in a number of other dicots.

You might want to take a look at these papers

Expression of intron modified NPTII genes in monocotyledonous and dicotyledonous
plant cells.
Mass, Simpson, Eckes, Schickler, Brown, Reiss, Salchert, Chet, Schell and
Reichel
Molecular Breeding, 3: 15-28, 1997

3' Splice Site Selection in Dicot Plant Nuclei is Position Dependent.
Lou, McCulough and Schuler
Molecular and Cellular Biology, Aug. 1993, p. 4485-4493

Construction of an intron-containing marker gene: splicing of the intron in
transgenic plants and its use in monitoring early events in Agrobacterium-
mediated plant transformation.
Vancanneyt, Schmidt, O'Connor-Sanchez, Willmitzer & Rocha-Sosa
Mol Gen Genet 220: 245-50 (1990)
_______________________________________________________________________________
Subject: Intron Processing
From:    yanwu at ksu.edu (Yan H Wu) at INTERNET
Date:    9/2/97  5:39 PM


>I would like to know whether intron sequences from one plant species can 
>always be recognized and processed in other plant species.  For instance, 
>can an Arabidopsis genomic clone with introns be correctly processed if 
>it is expressed in tobacco, tomato, potato, aspen or pine?  

>Thanks for help.

>Yan Wu





---------------------------------------------------------------------------
All comments/opinions in this message are solely those of the sender, 
and do not necessarily reflect the views of Epitope, Incorporated 
or any of its subsidiaries.
---------------------------------------------------------------------------




More information about the Methods mailing list